| Appearance: Molecular weight: CAS number: Molecular formula: | dark blue powder 556.18 1223357-57-0 C35H42ClN3O |
Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.
This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.
Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.
General properties
| Appearance: Molecular weight: CAS number: Molecular formula: Solubility: Quality control: | dark blue powder 556.18 1223357-57-0 C35H42ClN3O good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM) NMR 1H, HPLC-MS (95%) Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| Spectral properties | |
| Excitation maximum, nm: | 646 |
| ε, L⋅mol-1⋅cm-1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: CF280: | 0.03 0.04 |
