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Fluorescein-dT Phosphoramidite

分子式:

分子量:

外 观:

C79H89N6O17P

1425.57

灰白色粉末


产品特性:


       5'-荧光素亚磷酰胺不包含4,4'-二甲氧基三苯甲基(DMT)基团,并且只能在5'末端添加一次,从而终止合成。该产物是使用6-羧基荧光素衍生物制备的。四氯(TET-,六氯(HEX-和二氯-二甲氧基(JOE-荧光素亚磷酰胺的设计旨在利用现代DNA测序仪和遗传分析仪的多色检测功能。荧光素亚磷酰胺被设计为产生与先前使用异硫氰酸荧光素(FITC)制备的荧光素型结构相同的荧光素。我们的荧光素亚磷酰胺还包含DMT基团,可定量偶联。使用6-FAM制备的类似结构6-荧光素亚磷酰胺也可与6-荧光素丝氨醇亚磷酰胺一起获得。可以将荧光素-dT插入所需序列中,以替代dT残基。我们提供五种荧光素支持物。传统上,荧光素CPG用于在3'末端添加荧光素标记。使用6-FAM制备的类似结构的3'-6-荧光素)CPG,以及6-荧光脂丝氨酸CPG也已上市。我们还提供3'-6-FAMCPGFluorescein-dT CPG,它们都是6-羧基荧光素(6-FAM)的衍生物。两者都是单一异构体,并使用酰胺键,该酰胺键在裂解和脱保护过程中是稳定的,并且不允许异构体形成。 3'-6-FAMCPG可以有效阻止聚合酶延伸以及核酸外切酶消化3'-末端。荧光素-dT CPG允许这两种酶的活动继续进行。正常的切割和用氢氧化铵的脱保护很容易产生荧光素标记的寡核苷酸。

一般性质             

分子式:

分子量:

外  观:

储存条件:

C79H89N6O17P  

1425.57   

灰白色粉末    

- 20℃,置于阴凉干燥处  


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AF430 NHS ester

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AF488 NHS ester

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BDP 558/568 NHS ester

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BDP 564/570 NHS ester

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ROX NHS ester, 5-isomer

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ROX NHS ester, 6- isomer

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Sulfo-Cyanine3 NHS ester

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TAMRA NHS ester, 5-isomer

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Cyanine7 maleimide

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ROX maleimide, 5-isomer

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FAM hydrazide, 5-isomer

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BDP 558/568 carboxylic acid

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BDP 564/570 carboxylic acid

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BDP 581/591 carboxylic acid

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BDP 630/650 carboxylic acid

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BDP FL carboxylic acid

BDP FL carboxylic acid

BDP TMR carboxylic acid

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BDP TR carboxylic acid

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Coumarin 343 X carboxylic acid

Coumarin 343 X carboxylic acid

Cyanine7 carboxylic acid

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Pyrenebutyric acid

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Sulfo-Cyanine5.5 carboxylic acid

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Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 carboxylic acid

sulfo-Cyanine7 dicarboxylic acid

sulfo-Cyanine7 dicarboxylic acid

Sulfo-Cyanine7.5 carboxylic acid

Sulfo-Cyanine7.5 carboxylic acid

AF488 NHS ester

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Cyanine5 alkyne

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Cyanine 3 Phosphoramidite

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TAMRA-dT

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Fluorescein-dT Phosphoramidite

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5'-Biotin Phosphoramidite

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5'-Fluorescein Phosphoramidite

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PO-PRO-1

PO-PRO-1

YO-PRO-1

YO-PRO-1

TO-PRO-1

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PO-PRO-3

PO-PRO-3

YO-PRO-3

YO-PRO-3

TO-PRO-3

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POPO-1

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YOYO-1

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TOTO-1

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YOYO-3

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AT 590 NHS ester

AT 590 NHS ester

 AT Rho 12 NHS Ester

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AT 550 NHS ester

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Texas Red-X NHS Ester

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AF 594 NHS Ester

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VIC NHS Ester

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6-CR6G NHS Ester

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MB Succinimidyl ester

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